Responsiveness of the widely used cardiomyocyte cell platforms to simulated ischemia/reperfusion

Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main source(s): Research, Development and Innovation Office Hungary (NKFIA; NVKP-16-1-2016-0017 Heart Program OTKA-FK 134751) MTA-SE System Pharmacology Research Group, Department Pharmacotherapy, Semmelweis University, H-1089 Budapest, Ischemic heart disease is the leading cause death worldwide; therefore, development cardioprotective therapies currently a main focus research. Cultured cell lines primary cultures play an integral role in cardiovascular However, there are many limitations when using these models, including variations proliferation capacity, uncontrolled stress during isolation, poor reproducibility low translational value. The aim our study was to test responsiveness most widely used cardiomyocyte platforms simulated ischemia/reperfusion injury (SI/R) considering effect differentiation protocols. Human, rat mouse as well were for vitro viability assay with or without cells exposed normoxic protocols: ischemic period 6 hours differentiated cultures, while non-differentiated 16 ischemia followed by 2 reperfusion applied. duration determined from preliminary experiments. Viability measured calcein assay. In human AC16 H9C2 cardiac lines, significantly reduced (50%) 16h SI / 2h R contrast, HL-1 line not h SI. neonatal myocytes 6h caused significant (25%). iPSC-derived did result (10%) whereas (37%). Responsiveness different types SI/R ranged between 40-100%. We have shown that reperfusion. Differentiation protocols markedly affects their response Comparative analyses cardiomyocytes can provide good basis accurate design systems.